Identification and Localization of Myxococcus xanthus Porins and Lipoproteins

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Identification and Localization of Myxococcus xanthus Porins and Lipoproteins

Myxococcus xanthus DK1622 contains inner (IM) and outer membranes (OM) separated by a peptidoglycan layer. Integral membrane, β-barrel proteins are found exclusively in the OM where they form pores allowing the passage of nutrients, waste products and signals. One porin, Oar, is required for intercellular communication of the C-signal. An oar mutant produces CsgA but is unable to ripple or stim...

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Identification and localization of the Tgl protein, which is required for Myxococcus xanthus social motility.

Tgl protein is required for the production of the type IV pili found at a pole of the Myxococcus xanthus cell. These pili are essential for social motility. Evidence is presented that Tgl is a membrane protein, based on experiments with polyclonal antibody specific for Tgl that was raised against the fusion proteins beta-galactosidase-Tgl and TrpE-Tgl. Immunoaffiity-purified antibody reacted wi...

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Identification and characterization of the Myxococcus xanthus argE gene.

The chromosomal acetylornithine deacetylase (argE) gene of Myxococcus xanthus was identified via homology to acetylornithine deacetylases from other bacterial species. A mutant carrying a disruption in argE was unable to grow on minimal media lacking supplemental arginine and formed fruiting bodies and spores in response to arginine starvation at high cell density.

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Identification of enhancer binding proteins important for Myxococcus xanthus development.

Enhancer binding proteins (EBPs) control the temporal expression of fruiting body development-associated genes in Myxococcus xanthus. Eleven previously uncharacterized EBP genes were inactivated. Six EBP gene mutations produced minor but reproducible defects in fruiting body development. One EBP gene mutation that affected A-motility produced strong developmental defects.

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Vegetative Cells and Microcysts of Myxococcus xanthus

A number of reports have demonstrated that microcysts, the resting cells produced by most myxobacteria, are more resistant than the corresponding vegetative cells. Baur (1) showed that mycrocysts of Myxococcus ruber withstood desiccation and wet and dry heat better than did vegetative cells. In 1936, Imshenetsky and Solnetzewa (6) reported comparable findings for Cytophaga hutchinsonil and C. e...

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ژورنال

عنوان ژورنال: PLoS ONE

سال: 2011

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0027475